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Department
  • Chemical Engineering and Biotechnology
    • Research Theme Lifelong Brain Development and Brain Ageing Neurons, Circuits and Networks Beyond the Neuron
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    Professor Clemens Kaminski

    University Position
    Professor

    Interests

    We develop advanced microscopic imaging techniques that permit us to elucidate molecular mechanisms of neurodegeneration. We use techniques such as lifetime, spectrum and polarisation resolved imaging that inform on protein misfolding, aggregation and interaction in live cells and organisms. Recently we have been able to follow the aggregation of Alpha synuclein in a C elegans model of Parkinson's disease and were able to correlate the appearance of aggregates with toxic phenotypes. We have also set up an optical superresolution microscope in Cambridge, based on dSTORM (direct Stochastic Optical Reconstruction Microscopy) and this permits us to visualise morphology and size of toxic oligomers at a resolution down to 10 nm.

    Key Publications

    Mutation of the ALS/FTD-associated RNA-binding protein FUS affects axonal development.

    DOI: http://doi.org/10.1523/JNEUROSCI.2148-23.2024
    Journal: J Neurosci
    E-pub date: 3 Jul 2024
    Authors: FWV Tartwijk, LCS Wunderlich, I Mela, S Makarchuk, MAH Jakobs, S Qamar, K Franze, GSK Schierle, PHS George-Hyslop, JQ Lin, CE Holt, CF Kaminski

    Protein Condensation, Cellular Organization, and Spatiotemporal Regulation of Cytoplasmic Properties.

    DOI: http://doi.org/10.1002/adbi.202101328
    Journal: Adv Biol (Weinh)
    E-pub date: 1 Nov 2022
    Authors: FW van Tartwijk, CF Kaminski

    The structure and global distribution of the endoplasmic reticulum network are actively regulated by lysosomes.

    DOI: http://doi.org/10.1126/sciadv.abc7209
    Journal: Sci Adv
    E-pub date: 1 Dec 2020
    Authors: M Lu, FW van Tartwijk, JQ Lin, W Nijenhuis, P Parutto, M Fantham, CN Christensen, E Avezov, CE Holt, A Tunnacliffe, D Holcman, L Kapitein, GSK Schierle, CF Kaminski

    Single particle trajectories reveal active endoplasmic reticulum luminal flow.

    DOI: http://doi.org/10.1038/s41556-018-0192-2
    Journal: Nat Cell Biol
    E-pub date: 1 Oct 2018
    Authors: D Holcman, P Parutto, JE Chambers, M Fantham, LJ Young, SJ Marciniak, CF Kaminski, D Ron, E Avezov

    Phase Separation of FUS is Modulated by Methylation State of Cation-π Interactions and Interaction with TNPO1

    DOI: http://doi.org/10.1016/j.cell.2018.03.056
    Journal: Cell
    E-pub date: 19 Apr 2018
    Authors: PH St George-Hyslop, S Qamar, G Wang, SJ Randle, FS Ruggeri, J Varela, CF Kaminski, GS Kaminski, M Vendruscolo, TPJ Knowles, D Klenerman, CE Holt, Q Lin, W meadows

    Single Molecule Translation Imaging Visualizes the Dynamics of Local β-Actin Synthesis in Retinal Axons.

    DOI: http://doi.org/10.1038/s41598-017-00695-7
    Journal: Sci Rep
    E-pub date: 6 Apr 2017
    Authors: F Ströhl, JQ Lin, RF Laine, HH-W Wong, V Urbančič, R Cagnetta, CE Holt, CF Kaminski

    ALS/FTD Mutation-Induced Phase Transition of FUS Liquid Droplets and Reversible Hydrogels into Irreversible Hydrogels Impairs RNP Granule Function.

    DOI: http://doi.org/10.1016/j.neuron.2015.10.030
    Journal: Neuron
    E-pub date: 18 Nov 2015
    Authors: T Murakami, S Qamar, JQ Lin, GSK Schierle, E Rees, A Miyashita, AR Costa, RB Dodd, FTS Chan, CH Michel, D Kronenberg-Versteeg, Y Li, S-P Yang, Y Wakutani, W Meadows, RR Ferry, L Dong, GG Tartaglia, G Favrin, W-L Lin, DW Dickson, M Zhen, D Ron, G Schmitt-Ulms, PE Fraser, NA Shneider, C Holt, M Vendruscolo, CF Kaminski, P St George-Hyslop

    Publications

    ERnet: a tool for the semantic segmentation and quantitative analysis of endoplasmic reticulum topology.

    DOI: http://doi.org/10.1038/s41592-023-01815-0
    Journal: Nat Methods
    E-pub date: 1 Apr 2023
    Authors: M Lu, CN Christensen, JM Weber, T Konno, NF Läubli, KM Scherer, E Avezov, P Lio, AA Lapkin, GS Kaminski Schierle, CF Kaminski

    High-throughput super-resolution single-particle trajectory analysis reconstructs organelle dynamics and membrane reorganization.

    DOI: http://doi.org/10.1016/j.crmeth.2022.100277
    Journal: Cell Rep Methods
    E-pub date: 22 Aug 2022
    Authors: P Parutto, J Heck, M Lu, C Kaminski, E Avezov, M Heine, D Holcman

    Intracellular Aβ42 aggregation leads to cellular thermogenesis

    DOI: http://doi.org/10.1101/2022.03.30.486355
    Journal:
    E-pub date: 1 Aug 2022
    Authors: CW Chung, A Stephens, T Konno, E Ward, E Avezov, C Kaminski, A Hassanali, G Kaminski Schierle

    Intracellular Aβ42 Aggregation Leads to Cellular Thermogenesis.

    DOI: http://doi.org/10.1021/jacs.2c03599
    Journal: J Am Chem Soc
    E-pub date: 8 Jun 2022
    Authors: CW Chung, AD Stephens, T Konno, E Ward, E Avezov, CF Kaminski, AA Hassanali, GS Kaminski Schierle

    Z-α1-antitrypsin polymers impose molecular filtration in the endoplasmic reticulum after undergoing phase transition to a solid state.

    DOI: http://doi.org/10.1126/sciadv.abm2094
    Journal: Sci Adv
    E-pub date: 8 Apr 2022
    Authors: JE Chambers, N Zubkov, M Kubánková, J Nixon-Abell, I Mela, S Abreu, M Schwiening, G Lavarda, I López-Duarte, JA Dickens, T Torres, CF Kaminski, LJ Holt, E Avezov, JA Huntington, PS George-Hyslop, MK Kuimova, SJ Marciniak

    Advances in the study of organelle interactions and their role in neurodegenerative diseases enabled by super-resolution microscopy.

    DOI: http://doi.org/10.1016/j.nbd.2021.105475
    Journal: Neurobiol Dis
    E-pub date: 1 Nov 2021
    Authors: M Lu, E Ward, FW van Tartwijk, CF Kaminski

    SPtsAnalysis: a high-throughput super-resolution single particle trajectory analysis to reconstruct organelle dynamics and membrane re-organization

    DOI: http://doi.org/10.1101/2021.09.18.460892
    Journal:
    E-pub date: 1 Aug 2021
    Authors: P Parutto, J Heck, M Lu, C Kaminski, M Heine, E Avezov, D Holcman

    A waveguide imaging platform for live-cell TIRF imaging of neurons over large fields of view.

    DOI: http://doi.org/10.1002/jbio.201960222
    Journal: J Biophotonics
    E-pub date: 1 Jun 2020
    Authors: IS Opstad, F Ströhl, M Fantham, C Hockings, O Vanderpoorten, FW van Tartwijk, JQ Lin, J-C Tinguely, FT Dullo, GS Kaminski-Schierle, BS Ahluwalia, CF Kaminski

    A protocol for single-molecule translation imaging in xenopus retinal ganglion cells

    DOI: http://doi.org/10.1007/978-1-0716-0532-5_14
    Journal:
    E-pub date: 1 Jan 2020
    Authors: F Ströhl, JQ Lin, FW van Tartwijk, HHW Wong, CE Holt, CF Kaminski

    On-Site Ribosome Remodeling by Locally Synthesized Ribosomal Proteins in Axons.

    DOI: http://doi.org/10.1016/j.celrep.2019.11.025
    Journal: Cell Rep
    E-pub date: 10 Dec 2019
    Authors: T Shigeoka, M Koppers, HH-W Wong, JQ Lin, R Cagnetta, A Dwivedy, J de Freitas Nascimento, FW van Tartwijk, F Ströhl, J-M Cioni, J Schaeffer, M Carrington, CF Kaminski, H Jung, WA Harris, CE Holt

    Receptor-specific interactome as a hub for rapid cue-induced selective translation in axons.

    DOI: http://doi.org/10.7554/eLife.48718
    Journal: Elife
    E-pub date: 20 Nov 2019
    Authors: M Koppers, R Cagnetta, T Shigeoka, LC Wunderlich, P Vallejo-Ramirez, J Qiaojin Lin, S Zhao, MA Jakobs, A Dwivedy, MS Minett, A Bellon, CF Kaminski, WA Harris, JG Flanagan, CE Holt

    Single Particle Trajectories Reveal Active Endoplasmic Reticulum Luminal Flow

    DOI: http://doi.org/10.1016/j.bpj.2018.11.964
    Journal: BIOPHYSICAL JOURNAL
    E-pub date: 1 Aug 2019
    Authors: P Parutto, JE Chambers, M Fantham, L Young, S Marciniak, CF Kaminski, D Ron, D Holcman, E Avezov

    RNA Docking and Local Translation Regulate Site-Specific Axon Remodeling In Vivo.

    DOI: http://doi.org/10.1016/j.neuron.2017.07.016
    Journal: Neuron
    E-pub date: 16 Aug 2017
    Authors: HH-W Wong, JQ Lin, F Ströhl, CG Roque, J-M Cioni, R Cagnetta, B Turner-Bridger, RF Laine, WA Harris, CF Kaminski, CE Holt

    α-Synuclein – Regulator of Exocytosis, Endocytosis, or Both?

    DOI: http://doi.org/10.1016/j.tcb.2017.02.002
    Journal: Trends Cell Biol
    E-pub date: 1 Jul 2017
    Authors: J Lautenschläger, CF Kaminski, GS Kaminski Schierle

    TriPer, an optical probe tuned to the endoplasmic reticulum tracks changes in luminal H2O2.

    DOI: http://doi.org/10.1186/s12915-017-0367-5
    Journal: BMC Biol
    E-pub date: 27 Mar 2017
    Authors: EP Melo, C Lopes, P Gollwitzer, S Lortz, S Lenzen, I Mehmeti, CF Kaminski, D Ron, E Avezov

    Investigating State Restriction in Fluorescent Protein FRET Using Time-Resolved Fluorescence and Anisotropy.

    DOI: http://doi.org/10.1021/acs.jpcc.6b11235
    Journal: J Phys Chem C Nanomater Interfaces
    E-pub date: 26 Jan 2017
    Authors: TS Blacker, W Chen, E Avezov, RJ Marsh, MR Duchen, CF Kaminski, AJ Bain

    TriPer, an optical probe tuned to the endoplasmic reticulum tracks H2O2 consumption by glutathione

    DOI: http://doi.org/10.1101/088351
    Journal:
    E-pub date: 1 Aug 2016
    Authors: EP Melo, C Lopes, P Gollwitzer, S Lortz, S Lenzen, I Mehmeti, C Kaminski, D Ron, E Avezov

    A method to quantify FRET stoichiometry with phasor plot analysis and acceptor lifetime ingrowth.

    DOI: http://doi.org/10.1016/j.bpj.2015.01.012
    Journal: Biophys J
    E-pub date: 10 Mar 2015
    Authors: W Chen, E Avezov, SC Schlachter, F Gielen, RF Laine, HP Harding, F Hollfelder, D Ron, CF Kaminski

    Retarded PDI diffusion and a reductive shift in poise of the calcium depleted endoplasmic reticulum.

    DOI: http://doi.org/10.1186/s12915-014-0112-2
    Journal: BMC Biol
    E-pub date: 10 Jan 2015
    Authors: E Avezov, T Konno, A Zyryanova, W Chen, R Laine, A Crespillo-Casado, EP Melo, R Ushioda, K Nagata, CF Kaminski, HP Harding, D Ron

    Lifetime imaging of a fluorescent protein sensor reveals surprising stability of ER thiol redox.

    DOI: http://doi.org/10.1083/jcb.201211155
    Journal: J Cell Biol
    E-pub date: 15 Apr 2013
    Authors: E Avezov, BCS Cross, GS Kaminski Schierle, M Winters, HP Harding, EP Melo, CF Kaminski, D Ron

    Protein amyloids develop an intrinsic fluorescence signature during aggregation.

    DOI: http://doi.org/10.1039/c3an36798c
    Journal: Analyst
    E-pub date: 7 Apr 2013
    Authors: FTS Chan, GS Kaminski Schierle, JR Kumita, CW Bertoncini, CM Dobson, CF Kaminski

    Analysis of the native structure, stability and aggregation of biotinylated human lysozyme.

    DOI: http://doi.org/10.1371/journal.pone.0050192
    Journal: PLoS One
    E-pub date: 1 Aug 2012
    Authors: M Ahn, E De Genst, GS Kaminski Schierle, M Erdelyi, CF Kaminski, CM Dobson, JR Kumita

    Towards multiparametric fluorescent imaging of amyloid formation: studies of a YFP model of alpha-synuclein aggregation.

    DOI: http://doi.org/10.1016/j.jmb.2009.10.066
    Journal: J Mol Biol
    E-pub date: 22 Jan 2010
    Authors: TJ van Ham, A Esposito, JR Kumita, S-TD Hsu, GS Kaminski Schierle, CF Kaminski, CM Dobson, EAA Nollen, CW Bertoncini