Research Focus


  • Axon Degeneration
  • Axon & Neuronal Degeneration
  • Axon Loss
  • Clinical Conditions

  • Motor neurone disease
  • Hereditary spastic paraplegia
  • Other 
  • Equipment & Techniques

  • Cell culture
  • Enzyme assays
  • Fluorescence microscopy
  • Immunohistochemistry
  • Recombinant protein expression
  • Back

    Dr Jonathan Gilley

    University Position
    Senior Research Associate


    My principle research area is investigation of the molecular mechanisms involved in axon degeneration after injury and in disease.

    Key Publications

    Enrichment of SARM1 alleles encoding variants with constitutively hyperactive NADase in patients with ALS and other motor nerve disorders.

    Journal: Elife
    E-pub date: 19 Nov 2021
    Authors: J Gilley, O Jackson, M Pipis, MA Estiar, A Al-Chalabi, MC Danzi, KR van Eijk, SA Goutman, MB Harms, H Houlden, A Iacoangeli, J Kaye, L Lima, Queen Square Genomics, J Ravits, GA Rouleau, R Schüle, J Xu, S Züchner, J Cooper-Knock, Z Gan-Or, MM Reilly, MP Coleman

    Homozygous NMNAT2 mutation in sisters with polyneuropathy and erythromelalgia.

    Journal: Exp Neurol
    E-pub date: 1 Oct 2019
    Authors: P Huppke, E Wegener, J Gilley, C Angeletti, I Kurth, JPH Drenth, C Stadelmann, A Barrantes-Freer, W Brück, H Thiele, P Nürnberg, J Gärtner, G Orsomando, MP Coleman

    Severe biallelic loss-of-function mutations in nicotinamide mononucleotide adenylyltransferase 2 (NMNAT2) in two fetuses with fetal akinesia deformation sequence.

    Journal: Exp Neurol
    E-pub date: 1 Oct 2019
    Authors: M Lukacs, J Gilley, Y Zhu, G Orsomando, C Angeletti, J Liu, X Yang, J Park, RJ Hopkin, MP Coleman, RG Zhai, RW Stottmann


    SARM1 is a multi-functional NAD(P)ase with prominent base exchange activity, all regulated bymultiple physiologically relevant NAD metabolites.

    Journal: iScience
    E-pub date: 18 Feb 2022
    Authors: C Angeletti, A Amici, J Gilley, A Loreto, AG Trapanotto, C Antoniou, E Merlini, MP Coleman, G Orsomando

    Sarm1 haploinsufficiency or low expression levels after antisense oligonucleotides delay programmed axon degeneration.

    Journal: Cell Rep
    E-pub date: 14 Dec 2021
    Authors: SA Gould, J Gilley, K Ling, P Jafar-Nejad, F Rigo, M Coleman

    Neurotoxin-mediated potent activation of the axon degeneration regulator SARM1.

    Journal: Elife
    E-pub date: 6 Dec 2021
    Authors: A Loreto, C Angeletti, W Gu, A Osborne, B Nieuwenhuis, J Gilley, E Merlini, P Arthur-Farraj, A Amici, Z Luo, L Hartley-Tassell, T Ve, LM Desrochers, Q Wang, B Kobe, G Orsomando, MP Coleman

    NAD+ cleavage activity by animal and plant TIR domains in cell death pathways.

    Journal: Science
    E-pub date: 23 Aug 2019
    Authors: S Horsefield, H Burdett, X Zhang, MK Manik, Y Shi, J Chen, T Qi, J Gilley, J-S Lai, MX Rank, LW Casey, W Gu, DJ Ericsson, G Foley, RO Hughes, T Bosanac, M von Itzstein, JP Rathjen, JD Nanson, M Boden, IB Dry, SJ Williams, BJ Staskawicz, MP Coleman, T Ve, PN Dodds, B Kobe

    Low levels of NMNAT2 compromise axon development and survival.

    Journal: Hum Mol Genet
    E-pub date: 1 Feb 2019
    Authors: J Gilley, PR Mayer, G Yu, MP Coleman

    Sarm1 Deletion, but Not WldS, Confers Lifelong Rescue in a Mouse Model of Severe Axonopathy.

    Journal: Cell Rep
    E-pub date: 3 Oct 2017
    Authors: J Gilley, RR Ribchester, MP Coleman

    NMN Deamidase Delays Wallerian Degeneration and Rescues Axonal Defects Caused by NMNAT2 Deficiency In Vivo.

    Journal: Curr Biol
    E-pub date: 20 Mar 2017
    Authors: M Di Stefano, A Loreto, G Orsomando, V Mori, F Zamporlini, RP Hulse, J Webster, LF Donaldson, M Gering, N Raffaelli, MP Coleman, J Gilley, L Conforti

    A rise in NAD precursor nicotinamide mononucleotide (NMN) after injury promotes axon degeneration.

    Journal: Cell Death Differ
    E-pub date: 1 May 2015
    Authors: M Di Stefano, I Nascimento-Ferreira, G Orsomando, V Mori, J Gilley, R Brown, L Janeckova, ME Vargas, LA Worrell, A Loreto, J Tickle, J Patrick, JRM Webster, M Marangoni, FM Carpi, S Pucciarelli, F Rossi, W Meng, A Sagasti, RR Ribchester, G Magni, MP Coleman, L Conforti

    Absence of SARM1 rescues development and survival of NMNAT2-deficient axons.

    Journal: Cell Rep
    E-pub date: 31 Mar 2015
    Authors: J Gilley, G Orsomando, I Nascimento-Ferreira, MP Coleman

    Wallerian degeneration: an emerging axon death pathway linking injury and disease.

    Journal: Nat Rev Neurosci
    E-pub date: 1 Jun 2014
    Authors: L Conforti, J Gilley, MP Coleman

    Rescue of peripheral and CNS axon defects in mice lacking NMNAT2.

    Journal: J Neurosci
    E-pub date: 14 Aug 2013
    Authors: J Gilley, R Adalbert, G Yu, MP Coleman